In Vitro Assessment of Myorelaxant and Antispasmodic Effects of Stigmas, Tepals, and Leaves Hydroethanolic Extracts of Crocus sativus

The valorization of Crocus sativus focuses mainly on the plant’s stigma because it is one most expensive and valuable spices in world has great value food, cosmetics, pharmacological industries. Due to this high value, other parts plant are considered as by-products; our study aimed evaluate myorelaxant antispasmodic activities byproducts (tepals leaves) compare them with plant. To investigate isolated rabbit rat jejunum, we used an vitro technic organ bath isotonic transducer. Our results showed that STG (hydroethanolic extract stigmas) LV had a moderate effect IC50 = 6.61 ± 1.5 5.08 0.45 mg/ml, respectively. TPL tepals) significant ( p ˂ 0.001 ) inhibitory amplitude jejunum basic contractions 1.36 0.15 mg/ml. also caused id="M2"> activity depending dose contraction induced by CCh (10−6 M) KCl (25 mM). slightly altered presence nifedipine percentage 26.8%. This difference statistically id="M3"> 0.01 ). Therefore, could induce L-type voltage-dependent Ca2+ channels, but not guanylate cyclase nitric oxide pathways. That confirmed comparable verapamil. HPLC-DAD analysis various C. shows three extracts contain kaempferol flavanol compound, was revealed be rich carotenoids crocin, these isomers trans cis-crocin, safranal, picrocrocin. In contrast, isorhamnetin quercetin, hesperidin mangiferin. conclusion, supports traditional use treat digestive problems will allow us explore future possibilities for treating bowel spasms using natural molecules derived from saffron.